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Latent TGFβ binding protein-1 and fibrillin-1 in human capsular opacification and in cultured lens epithelial cells

机译：潜在的TGFβ结合蛋白-1和原纤维蛋白-1在人包膜混浊和晶状体上皮细胞培养中的作用

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BACKGROUND/AIM—It was previously reported that collagenous extracellular matrix (ECM) in human capsular opacification contained isoforms of transforming growth factor β (TGFβ). In the present study, the authors performed immunohistochemistry to examine whether ECM in human capsular opacification and in cultures of bovine lens epithelial cells (LECs) contained latent TGFβ binding protein-1 (LTBP-1), TGFβ1 latency associated peptide (β1-LAP), and fibrillin-1, a suspected ligand of LTBP-1 as well as a component of the extracellular microfibrillar apparatus. The aim of the study was to further clarify the mechanism of TGFβ1 deposition in ECM of capsular opacification.
METHODS—Human capsular opacification specimens and uninjured lens capsules, as well as cultured bovine LECs, were processed for immunohistochemistry using antibodies against LTBP-1, β1-LAP, fibrillin-1, and collagen type I.
RESULTS—LTBP-1, β1-LAP, and fibrillin-1 all were localised to the ECM in human capsular opacification. Uninjured lens epithelium stained for β1-LAP, but not for LTBP-1 and fibrillin-1. ECM deposited in confluent LEC cultures stained for LTBP-1, β1-LAP, and fibrillin-1, while cultures with only sparse cellularity were unstained for LTBP-1 or fibrillin-1.
CONCLUSIONS—LECs upregulate LTBP-1 and fibrillin-1 during postoperative healing. LTBP-1, β1-LAP, and fibrillin-1 colocalised to the ECM in capsular opacification and in confluent LEC cultures. TGFβ1 is considered to deposit in ECM in the large latent form. ECM secreted by LEC may function as a scavenger or repository of TGFβ.


机译：背景/目的-先前曾报道人荚膜混浊中的胶原细胞外基质（ECM）包含转化生长因子β（TGFβ）的同工型。在本研究中，作者进行了免疫组织化学检查，以检查人包膜混浊和牛晶状体上皮细胞（LEC）培养物中的ECM是否含有潜在的TGFβ结合蛋白-1（LTBP-1），TGFβ1潜伏期相关肽（β1-LAP）以及原纤维蛋白1（一种可能是LTBP-1的配体）以及细胞外微原纤维装置的组成部分。该研究的目的是进一步阐明TGFβ1在囊膜混浊的ECM中沉积的机制。方法—使用针对LTBP-1，β1-LAP，原纤维蛋白-1和I型胶原的抗体对人的囊混浊标本和未受伤的晶状体囊以及培养的牛LEC进行免疫组织化学处理。结果—LTBP-1，β1-在人包膜混浊中，LAP和原纤维蛋白1均定位于ECM。未损伤的晶状体上皮对β1-LAP染色，但对LTBP-1和原纤维蛋白1则不染色。 ECM沉积在融合的LEC培养物中，对LTBP-1，β1-LAP和原纤维蛋白1染色，而仅具有稀疏细胞性的培养物未对LTBP-1或原纤维蛋白1染色。结论—LEC在术后愈合过程中上调了LTBP-1和原纤维蛋白-1。在荚膜混浊和融合的LEC培养物中，LTBP-1，β1-LAP和原纤维蛋白1共定位于ECM。 TGFβ1被认为以大潜在形式沉积在ECM中。 LEC分泌的ECM可能充当TGFβ的清除剂或储存库。

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